A full suite rapid lateral flow devices for common food allergens, OnSite® test kits are suitable for commercial kitchens, food and beverage manufacturers, auditing professionals, and anyone dedicated to allergen safety.
The OnSite® line of rapid lateral flow test kits is split into three interrelated categories:
This test kit is designed to detect gluten from wheat, barley, and rye in a variety of baked and raw foods (solids, powders, and liquids) and on environmental surfaces.
OnSite® Gluten (PA-44) has a detection limit of 11 µg/100 cm² gluten on surfaces. The LOD can be variably adjusted to 5, 10, or 20 mg/kt (ppm) gluten in food products, depending on the matrix and the user’s individual needs.
The assay is intended for laboratory and industry use, including within food production facilities, commercial kitchens, contract laboratories, and auditing programs. Testing should only be performed by trained personnel.
AOAC-PTM License # 012501 was granted for the matrices of: rice flour, oat flour, spice mix, baked bread, and stainless steel (certificate and validation data available on request).
OnSite® Gluten is suitable for general use in the food industry. It can detect barley, wheat, and rye gluten in a wide variety of raw and processed foods and on a variety of working surfaces. The kit uses the robust 2D4 antibody, which has similar characteristics to the R5 antibody and an increased ability to detect deamidated gluten.
The kit holds AOAC-PTM Certification for the following matrices: baked bread, rice flour, oat flour, spice mix, and stainless steel.
It has also been validated against a variety of commodities and common food ingredients; there are no known cross-reactive commodities.
Like other rapid antibody-based lateral flow tests, OnSite® Gluten is not designed for fermented or hydrolyzed gluten. It may show reduced sensitivity when testing matrices high in fats or polyphenolic compounds.
Rapid testing is an important component of any food safety plan. Using rapid tests can provide additional reassurance as to the gluten-free status of incoming ingredients, validate the efficacy of a food production facility’s allergen cleaning protocol, and verify that those cleaning protocols have been undertaken successfully and safely.
The 2D4 antibody in OnSite Gluten has a unique ability to detect deamidated gluten. But what IS deamidated gluten and why does it matter?
Deamidation is a chemical reaction that modifies protein structure. Sometimes it is done intentionally, to improve the functionality of gluten as a food ingredient. For example, wheat gluten is sometimes used as a binder in meat products or a means to increase protein content in foods. Sometimes deamidation is a consequence of the baking or manufacturing process (heat and acid can deamidate gluten).
Lastly, deamidation happens naturally in the body — it is a part of digestion. People who have undergone a celiac blood panel may recall having their levels of deamidated gluten tested.
Studies indicate that deamidated gluten may be more immunotoxic to people with celiac disease. Studies also indicate that this form of gluten is very hard for traditional tests to detect: a 2010 study in the Journal of Cereal Science found that:
Random deamidation decreased the intensities to 13–54% of the intensity obtained for the intact peptides. Deamidation representing the transglutaminase deamidation decreased the intensities to 4–8%. Deamidation of gluten proteins abolished the recognition by omega-gliadin and G12 antibodies and decreased the recognition of R5 by 600 times when analyzed by the sandwich method and 125 times by the competitive method.
Using the OnSite Gluten Test Kit is a simple way to ensure deamidated gluten is correctly monitored.
Test kits are shelf stable (between 2° and 30°C or 36-80°F) for a minimum of four months. However, if stored between 2°-8°C, in the refrigerator, shelf life is > 12 months.
We recommend storing in the refrigerator to preserve kit shelf life and sensitivity. However, please ensure kits are brought to room temperature before using.
Complete Instructions for Use are included in each test kit. Tests are simple to run and no previous knowledge is required.
However, our team can set up a video training call at your discretion.
Contact your distributor for more details.
No. While an LFD strip reader can be used to ensure consistent, electronic results, there is no way to reliably quantify the gluten from this kit.
In situations where a precise value is required, ELISA analysis should be undertaken by trained personnel.
These simple, robust lateral flow devices quickly and accurately detect traces of 17 different food allergens.
OnSite® III Allergen test kits at a glance:
OnSite® III Allergen Test Kits can be ordered for any of the following analytes:
* gluten kit requires additional supplies and separate extraction protocol.
OnSite® III Allergen can be used on raw ingredients, finished products, rinsewater, and surfaces. Analyzing surfaces does require the separate purchase of OnSite® Accessory kit. OnSite® Allergen Multiplex Tree Nut cannot be used to analyze foods.
OnSite® III Allergen kits are each designed to react to a specific allergen. For environments where multiple allergens may be present, kits may be combined as needed. With the exception of gluten, all allergens share a common extraction protocol. This means that one extracted sample can be used to detect several allergens at the same time.
Test kits should be kept between 2°-8°C, in the refrigerator. This preserves kit shelf life and sensitivity.
Complete Instructions for Use are included in each test kit. Tests are simple to run and no previous knowledge is required. Contact your distributor for more details.
No. While the three-line design does distinguish between low and high levels of allergen, a precise value is not possible.
In situations where a precise value is required, ELISA analysis should be undertaken by trained personnel.
This convenient lateral flow test kit quickly detects traces of tree nut protein in rinsewater and on surfaces.
Allergen Multiplex Tree Nut at a glance:
Tree nut allergies are among the most common food allergies for both children and adults. Accordingly, many countries consider tree nuts to be a major food allergen requiring careful labeling and hazard management. And because many individuals with tree nut allergies are not allergic to all tree nuts but only to some, allergen legislation frequently requires each specific nut to be labeled and handled distinctly. For example, a person may need to avoid cashew and pistachio only — and so a product label must indicate the presence not just of “tree nuts” but also detail which nuts are included.
No. The OnSite® Allergen Multiplex Tree Nut test kit is targeted at the six most common tree nuts.
Definition of “tree nut” vary by country, but nearly always include more nut species than these six. If you require information about a nut other than almond, cashew, hazelnut, pecan, pistachio, or walnut — contact your distributor to see if an OnSite® III Allergen kit is available for your specific need.
OnSite® Multiplex Tree Nut is strictly for surfaces and rinsewater. It cannot be used to analyze foods.
In addition, OnSite® Multiplex Tree Nut is designed to provide a single “detected” or “not detected” signal when any or all of the target analytes are found in detectable quantity. This means that the presence of sufficient almond or cashew or hazelnut or pecan or pistachio or walnut — or any combination of those nuts — will trigger a positive (“detected”) result.
If more targeted information about any one specific allergen is needed, the relevant OnSite® III Allergen Test Kit can be used.
Test kits should be kept between 2°-8°C, in the refrigerator. This preserves kit shelf life and sensitivity.
Complete Instructions for Use are included in each test kit. Tests are simple to run and no previous knowledge is required. Contact your distributor for more details.
